Safer storge and transportation of samples,especially for biological samples,has been critical,owing to the risk of potentially hazardous substances. Therefore the containers should be complied with the transport regulations,such as those recommended by the Internation Civil Aviation Organization (ICAO) and the International Air transport association (IATA). These require the primary or secondary packing to be able to being equivalent to atmospheric pressure at sea leakage .95kPa without leakage. A pressure of 95kPa is0.95bar(1 bar being equivalent to atmospheric pressure at sea leakage.95kPa test is acording to ASTM D4991 Standard test method for leakage testing of empty rigid containers by vacuum method.